A selection of mother trees for seed stock is based on measurement parameters and visual characteristics without taking into account the susceptibility of the trees to root and butt rot disease. In areas formerly used for agriculture, the first generation of a pine stand can fail due to root pathogens of the genus Heterobasidion. The problem of root and butt rot remains one of the most important silvicultural problems in all European countries including Poland and Ukraine. One solution is to use tree progeny with increased disease resistance (survivability) in reforestation to create more sustainable forests for future generations. In continuation of a study on 20−year−old progenies of clonal seed orchards (CSO) and their controls from three regions of Ukraine (Eastern, Central and Western Ukraine), a comprehensive evaluation of CSO progenies was conducted and promising variants for cultivation in the local conditions of the Ukrainian left−bank forest−steppe were identified. For a comprehensive evaluation, a traditional assessment scale was used supplemented by a proposed proprietary scale based on previous phytopathological studies. The new population assessment method took into account not only growth intensity and condition indicators (diameter, height, quality and condition), but also factors of resistance to root pathogens (needles, xylem, latewood, resin).

Adamenko, T.I., 2014. Ahroklimatychne zonuvannya terytoriyi Ukrayiny z vrakhovannyam zminy klimatu. Available from: https://www.gwp.org/globalassets/global/gwp-cee_files/idmp-cee/idmp-agroclimatic.pdf.
Amaral, J., Valledor, L., Alves, A., Martín-García, J., Pinto, G., 2022. Studying tree response to biotic stress using a multi-disciplinary approach: The pine pitch canker case study. Frontiers in Plant Science, 13: 81-87. DOI: https://doi.org/10.3389/fpls.2022.916138.
Andreieva, V., Voitiuk, V., 2008. Taksatsiyno-selektsiyna otsinka pivsibsovykh potomstv sosny zvychaynoyi. Scientific Bulletin of UNFU, 18 (8): 30-36. Available from: https://nv.nltu.edu.ua/Archive/2008/18_8/index.htm.
Andreieva, V., Voitiuk, V., Kychyliuk, O., Hetmanchuk, A., 2020. Lisivnycho-selektsiyna otsinka pivsibsiv sosny zvychaynoyi u Volynskiy oblasti. (Forest-selection evaluation of half-sib posterities of Scots pine in the Volyn region). Lesya Ukrainka Eastern European National University Scientific Bulletin Series: Biological Sciences, 2 (390): 8-15. DOI: https://doi.org/10.29038/2617-4723-2020-390-2-8-15.
Beier, G., Lund, C,. Held, B., Ploetz, R., Konkol, J., Blanchette, R., 2021. Variation in xylem characteristics of botanical races of Persea americana and their potential influence on susceptibility to the pathogen Raffaelea lauricola. Tropical Plant Pathology, 46: 232-239. DOI: https://doi.org/10.1007/s40858-020-00397-y.
Berlin, M., Persson, T., Jansson, G., Haapanen, M., Ruotsalainen, S., Bärring, L., Andersson Gull, B., 2016. Scots pine transfer effect models for growth and survival in Sweden and Finland. Silva Fennica, 50 (2): 1-21. DOI: https://doi.org/10.14214/sf.1562.
Bert, D., Lasnier, J.B., Capdevielle, X., Dugravot, A., Desprez-Loustau, M.L., 2016. Powdery mildew decreases the radial growth of oak trees with cumulative and delayed effects over years. PLoS One, 11 (5): e0155,344. DOI: https://doi.org/10.1371/journal.pone.0155344.
Beuker, E., 1994. Long-term effects of temperature on the wood production of Pinus sylvestris L. and Picea abies (L.) Karst. in old provenance experiments. Scandinavian Journal of Forest Research, 9 (1-4): 34-45. DOI: https://doi.org/10.1080/02827589409382810.
Björkman, E., 1972. Die prüfung forstlicher baumarten auf resistenz gegen parasitäre pilze 1. European Journal of Forest Pathology, 2 (4): 229-237. DOI: https://doi.org/10.1111/j.1439-0329.1972.tb00366.x.
Cobb Jr, F., 1968. Inhibitory effects of volatile oleoresin components on Fomes annosus and four Ceratocystis species. Phytopathology, 58: 1327-1335.
Dasgupta, P., 2020. A review on defense responses and resistance breeding in forest tree species. International Journal of Innovative Research and Advanced Studies (IJIRAS), 7: 227-225.
Dinus, R.J., 1972. Testing for fusiform rust resistance in slash pine. In: R.T. Bingham, R.J. Hoff, G.I. McDonald, eds. Biology of rust resistance in forest trees. Washington: Department of Agriculture, Miscellaneous Publications, 1221: 331-339.
Durodola, B., Blumenstein, K., Terhonen, E., 2023. Genetic variation of Picea abies in response to the artificial inoculation of Heterobasidion parviporum. European Journal of Forest Research, 142 (2): 443-453. DOI: https://doi.org/10.1007/s10342-023-01534-3.
Dyshko, V., Torosova, L., 2016a. Osoblyvosti morfometrychnykh ta anatomichnykh oznak sosny zvychaynoyi (Pinys sulvestris Ll.) v urazhenomu korenevoyu hubkoyu nasadzhenni. [Peculiarities of morphometric and anatomical characteristics of Scots pine (Pinus sylvestris L.) in the stand affected by root rot]. Lisivnytstvo i Ahrolisomelioratsiya/Forestry and Forest Melioration, 129: 153-161. Available from: https://forestry-forestmelioration.org.ua/index.php/journal/issue/archive.
Dyshko, V., Torosova, L., 2016b. Osoblyvosti rostovykh protsesiv sosny zvychaynoyi u nasadzheni, urazhenomu korenevoyu hubkoyu. (Features of growth processes of Scots pine in plantations affected by root rot). Lisivnytstvo i Ahrolisomelioratsiya/Forestry and Forest Melioration, 128: 134-142. Available from: https://forestry-forestmelioration.org.ua/index.php/journal/article/view/154/140.
Dyshko, V., Torosova, L., 2017. Morfoloho-anatomichni kharakterystyky khvoyi potomstv pryrodnykh ta syntetychnykh populyatsiy sosny zvychaynoyi u sortovyprobnykh kul’turakh. (Morphological and anatomical characteristics of needles of natural and synthetic Scots pine progenies in the variety test). Lisivnytstvo i Ahrolisomelioratsiya/Forestry and Forest Melioration, 131: 78-86. Available from: https://forestry-forestmelioration.org.ua/index.php/journal/article/view/57/48.
Dyshko, V., Torosova, L., 2018. Kompleksne otsinyuvannya kandydativ u syntetychni sorty-populyatsiyi v sortovyprobnykh kul’turakh sosny zvychaynoyi v DP “Hutyans’ke lisove hospodarstvo”. (A comprehensive assessment of candidates to synthetic variety-populations in the Scots pine variety tests in Gutyanske Forest Enterprise). Lisivnytstvo i Ahrolisomelioratsiya/Forestry and Forest Melioration, 132: 57-65. DOI: https://doi.org/10.33220/1026-3365.132.2018.57.
Dyshko, V., Oszako, T., Borowik, P., 2023. Kompleksne otsinyuvannyapotomstv klonovykh nasinnykh plantatsiysosny zvychaynoyi za rostovymy kharakterystykamy ta oznakamy stiykosti v umovakh Lisostepu Kharkivs’koyi oblasti. (Comprehensive assessment of Scots pine clone seed orchard progenies by growth characteristics and resistance traits in the Forest-Steppe zone of Kharkiv region). Lisivnytstvo i Ahrolisomelioratsiya/Forestry and Forest Melioration, 143: 61-72. DOI: https://doi.org/10.33220/1026-3365.143.2023.61.
Franceschi, V.R., Krokene, P., Christiansen, E., Krekling, T., 2005. Anatomical and chemical defenses of conifer bark against bark beetles and other pests. New Phytologist, 167 (2): 353-376. DOI: https://doi.org/10.1111/j.1469-8137.2005. 01436.x.
Funda, T., El-Kassaby, Y.A., 2012. Seed orchard genetics. CABI Reviews, 2012: 1-23. DOI: https://doi.org/10.1079/pavsnsnr20127013.
Good, P.I., 2006. Resampling methods: A practical guide to data analysis. Boston: Birkhäuser, 218 pp. DOI: https://doi.org/10.1007/0-8176-4444-x.
Gori, Y., Camin, F., Cherubini, P., La Porta, N., 2011. Tree-ring as proxies of stress caused by Heterobasidion parviporum at three different mature stands in Trentino. In: XIII Conference ‘Root and Butt Rot of Forest Trees’ IUFRO Working Party 7.02.01, pp. 62-64. DOI: https://doi.org/10.13140/RG.2.1.3193.2964.
Harju, A.M., Venäläinen, M., Anttonen, S., Viitanen, H., Kainulainen, P., Saranpää, P., Vapaavuori, E., 2003. Chemical factors affecting the brown-rot decay resistance of scots pine heartwood. Trees, 17: 263-268. DOI: https://doi.org/10.1007/s00468-002-0233-z.
Hattemer, H., 1966. Geographic variation of residence to Lophodermium pinastri in Scots pine. In: H.D. Gerhold, E.J. Schreiner, R.E. Mcdermott, J.A. Winieski, eds. Breeding Pest-Resistant Trees. Pergamon Press, pp. 97-102. DOI: https://doi.org/10.1016/B978-0-08-011764-5.50028-6.
Hayda, Y., Los, S., Yatsyk, R., Tereshchenko, L., Shlonchak, G., Mytrochenko, V., Neyko, I., Samodai, V., Smashnyuk, L., Klisz, M., 2019. Seed orchards in Ukraine: Past, present and prospects for the future. Folia Forestalia Polonica, Series A – Forestry, 61 (4): 284-298. DOI: https://doi.org/10.2478/ffp-2019-0028.
Hietala, A.M., Eikenes, M., Kvaalen, H., Solheim, H., Fossdal, C.G., 2003. Multiplex real-time PCR for monitoring Heterobasidion annosum colonization in Norway spruce clones that differ in disease resistance. Applied and Environmental Microbiology, 69 (8): 4413-4420. DOI: https://doi.org/10.1128/AEM.69.8.4413-4420.2003.
Holmes, R.L., 1994. Dendrochronology program library user’s manual. Tucson: Laboratory of tree-ring research, University of Arizona, 51 pp.
Hordiyenko, M.I., Huz, M.M., Debryniuk, Yu.M., Maurer, V.M., 2005. Lisovi kultury. (Forest plantations). Lviv: Kamula, 608 pp.
Kinloch, B., 1972. Genetic variation in resistance to Cronartium and Peridermium rust in hard pines. In: R. Bingham, R. Hoff., G. McDonald, eds. Biology of Rust Resistance in Forest Trees, Proceedings of NATO-IUFRO Advanced Study Institute, Miscellaneous Publication no. 1221 (681). Washington: US Department of Agriculture and Forest Services, pp. 445-463.
Klepzig, K.D., Smalley, E.B., Raffa, K.F., 1995. Dendroctonus valens and Hylastes porculus (Coleoptera: Scolytidae): Vectors of pathogenic fungi (Ophiostomatales) associated with red pine decline disease. The Great Lakes Entomologist, 28 (1): 81-87. Available from: https://scholar.valpo.edu/tgle/vol28/iss1/5.
Korhonen, K., 1978. Intersterility groups of Heterobasidion annosum. Metsäntutkimuslaitoksen Julkaisuja – Communicationes Instituti Forestalis Fenniae, 94 (6): 1-25.
Korshikov, I., Demkovich, A., 2008. Geneticheskiye osobennosti ustoychivoy k kornevoy gubke sosny obyknovennoy v iskusstvennykh nasazhdeniyakh stepnoy zony Ukrainy. (Genotypic peculiarities of resistant to root fungus scotch pine plants in artificial stands of the Ukrainian steppe-zone). Tsitologiia i Genetika/Cytology and Genetics, 42 (5): 41-46. Available from: http://dspace.nbuv.gov.ua/handle/123456789/8222.
Lai, M., Dong, L., Yi, M., Sun, S., Zhang, Y., Fu, L., Xu, Z., Lei, L., Leng, C., Zhang, L., 2017. Genetic variation, heritability and genotype×environment interactions of resin yield, growth traits and morphologic traits for Pinus elliottii at three progeny trials. Forests, 8 (11): 409. DOI: https://doi.org/10.3390/f8110409.
Langner, W., 1952. Züchtung auf resistenz gegen Lophodermium pinastri bei Pinus sylvestris. Zeitschrift für Forstgenetik, 1: 59.
Lindgren, D., 2013. Seed orchards and aspects on supporting tree breeding. In: T. Fenning, ed. Challenges and Opportunities for the World’s Forests in the 21st Century. Forestry Sciences, vol 81. Dordrecht: Springer, pp. 481-487. DOI: https://doi.org/10.1007/978-94-007-7076-8_20.
Lombardero, M.J., Ayres, M.P., Ayres, B.D., 2006. Effects of fire and mechanical wounding on Pinus resinosa resin defenses, beetle attacks, and pathogens. Forest Ecology and Management, 225 (1-3): 349-358. DOI: https://doi.org/10.1016/j.foreco.2006.01.010.
Los, S., Tereshchenko, L., Gayda, Y., Ustimenko, P., Yatsyk, R., 2014. State of forest genetic resources in Ukraine. Kharkiv: Planeta-print, 139 pp. .
Los, S., Tereshchenko, L., Shlonchak, G., Samoday, V., Neyko, I., 2015. Rezul’taty vidboru plyusovykh derev sosny i dubav rivnynniy chastyni Ukrayiny ta v Krymu u 2010-2014 rr. (Results of pine and oak plus trees selection in the plains of Ukraine and in Crimea in 2010-2014). Lisivnytstvo i Ahrolisomelioratsiya/Forestry and Forest Melioration, 126: 139-147.
Los, S., Tereshchenko, L., Torosova, L., Hayda, Y.I., Vysotska, N.Y., Yatsyk, R.M., Grigorieva, V.G., Plotnikova, O.M., Shlonchak, G.A., Mitrochenko, V.V., Dyshko V.A., 2020. Metodyka sortovyprobuvannya lisovykh derevnykh porid. Vidomche vyprobuvannya. Kharkiv: Ukrainian Research Institute of Forestry and Forest Melioration.
Manly, B.F., Alberto, J., 2020. Randomization, bootstrap and Monte Carlo methods in biology. Texts in Statistical Science. Boca Raton: CRC press, 358 pp.
Marčiulynas, A., Sirgedaite·-Še·žiene·, V., Ţemaitis, P., Baliuckas, V., 2019. The resistance of Scots pine (Pinus sylvestris L.) half-sib families to Heterobasidion annosum. Forests, 10 (3): 287. DOI: https://doi.org/10.3390/f10030287.
Marčiulynas, A., Sirgedaite·-Še·žiene·, V., Ţemaitis, P., Jansons, A., Baliuckas, V., 2020. Resistance of Scots pine half-sib families to Heterobasidion annosum in progeny field trials. Silva Fennica, 54 (4): 10276. DOI: https://doi.org/10.14214/sf.10276.
Martinsson, O., 1979. Testing scots pine for resistance to Lophodermium needle cast. Studia Forestalia Suecica, 150: 54. Available from: http://urn.kb.se/resolve?urn= urn:nbn:se:slu:epsilon-9-48.
Matras, J., 2008. A review of the seed orchard programme in Poland. In: D. Lindgren, ed. Proceedings of a Seed Orchard Conferencet at Umeĺ, Umeĺ, Sweden, September 26-28, 2007, Umeĺ, Sweden, pp. 165-171. Available from: https://core.ac.uk/download/pdf/11695652.pdf#page=165.
Mbora, A., Lillesř, J.B., Schmidt, L., Angaine, P., Meso, M., Omondi, W., Ahenda, J., Mutua, N., Orwa, C., Jamnadass, R., 2009. Tree seed source reclassification manual. Nairobi: World Agroforestry Centre, 40 pp. Available from: https://www.worldagroforestry.org/sites/default/files/Tree_Seed_source_classification_manual.pdf.
McKeand, S., Jett, J., O’Berry, S., Heine, A., 2017. New challenges for seed orchard management of loblolly pine in the southern US. In: T. Funda, H. Hallingbäck, C. Almqvist, U. Wennström, eds. Proceedings of IUFRO Seed Orchard Conference 2017, p. 18.
Mukrimin, M., Kovalchuk, A., Ghimire, R.P., Kivimäenpää, M., Sun, H., Holopainen, J.K., Asiegbu, F.O., 2019. Evaluation of potential genetic and chemical markers for Scots pine tolerance against Heterobasidion annosum infection. Planta, 250: 1881-1895. DOI: https://doi.org/10.1007/s00425-019-03270-8.
Musienko, S., Luk’yanets, V., Tarnopylska, O., Kobets, O., Babenko, V., 2018. Merchantability and assortment structure of pine stands affected by root rot in the Volyn Polissya region, Ukraine. Central European Forestry Journal, 64 (2): 96-103. DOI: https://doi.org/10.1515/forj-2017-0034.
Mykhailichenko, O.A., Ustsky, I., Gnoevy, I. 2020. Vplyv korenevoyi hubky na rist, strukturu ta sanitarnyy stan sosnovykh nasadzhen, stvorenykh na staroornykh zemlyakh u lisostepu kharkivskoyi oblasti. (Influence of root rot on growth, structure and health condition of the pine stands established on formerly arable land in Forest-Steppe, Kharkiv Region). Lisivnytstvo i Ahrolisomelioratsiya/Forestry and Forest Melioration, 136: 172-183. DOI: https://doi.org/10.33220/1026-3365.136.2020.172.
Negrutskiy, S., 1986. Kornevaya gubka. Moscow: Agropromizdat, 196 pp.
Nikkanen, T., 2008. A review of Scots pine and Norway spruce seed orchards in Finland. In: Proceedings of a Seed Orchard Conferencet at Umeĺ, Umeĺ, Sweden, September 26-28, 2007, pp. 195-198. Available from: https://pub.epsilon.slu.se/3151/1/ZProcFinalFeb08.pdf#page=195.
Oliva, J., Stenlid, J., 2011. Validation of the rotstand model for simulating Heterobasidion annosum root rot in Picea abies stands. Forest Ecology and Management, 261 (11): 1841-1851. DOI: https://doi.org/10.1016/j.foreco.2011.02. 007.
Oszako, T., Kukina, O., Dyshko, V., Moser, W.K., Ślusarski, S., Okorski, A., Borowik, P. 2023. Afforestation of land abandoned by farmers poses threat to forest sustainability due to Heterobasidion spp. Forests, 14 (5): 954. DOI: https://doi.org/10.3390/f14050954.
Persson, B., Beuker, E., 1997. Distinguishing between the effects of changes in temperature and light climate using provenance trials with Pinus sylvestris in Sweden. Canadian Journal of Forest Research, 27 (4): 572-579. DOI: https://doi.org/10.1139/x96-22.
Polozhentsev, P., 1951. Metod iskusstvennykh raneniy dlya opredeleniya zhiznesposobnosti sosny. (The method of artificial wounds to determine the viability of pine). Lesovedeniye, 7: 26-29.
Prior, C., 1976. Resistance by Corsican pine to attack by Heterobasidion annosum. Annals of Botany, 40 (2): 261-279. DOI: https://doi.org/10.1093/oxfordjournals.aob.a085128.
Protasov, A., Zhurova, P., 1988. Osoblyvosti morfoloho-anatomichnoyi budovy vehetatyvnykh orhaniv siyantsiv sosny zvychaynoyi riznoho heohrafichnoho pokhodzhennya. (Peculiarities of the morphological and anatomical structure of the vegetative organs of common pine seedlings of various geographical origins) Lesovedeniye, 2: 85-88.
Rieksts-Riekstinš, R., Zeltinš, P., Baliuckas, V., Bruna, L., Zaluma, A., Kapostinš, R., 2019. Pinus sylvestris breeding for resistance against natural infection of the fungus Heterobasidion annosum. Forests, 11 (1): 23. DOI: https://doi.org/10.3390/f11010023.
Škipars, V., Baumanis, I., Rungis, D., 2010. Research on genetic aspects of scots pine resistance to root rot. In: Proceedings of Annual 16th International Scientific Conference Research for Rural Development 2010, Latvia University of Agriculture, pp. 222-226. Available from: https://llufb.llu.lv/conference/Research-for-Rural-Development/2010/LatviaResearchRuralDevel2010-16th_volume1.pdf#page=222.
Skrřppa, T., Solheim, H., Hietala, A., 2015a. Variation in phloem resistance of Norway spruce clones and families to Heterobasidion parviporum and Ceratocystis polonica and its relationship to phenology and growth traits. Scandinavian Journal of Forest Research, 30 (2): 103-111. DOI: https://doi.org/10.1080/02827581.2014.963144.
Skrřppa, T., Solheim, H., Steffenrem, A., 2015b. Genetic variation, inheritance patterns and parent-offspring relationships after artificial inoculations with Heterobasidion parviporum and Ceratocystis polonica in Norway spruce seed orchards and progeny tests. Silva Fennica, 49 (1): 1191. DOI: https://doi.org/10.14214/sf.1191.
Song, Y., Bouteiller, X.P., Larter, M., Plomion, C., Sin, F., Delzon, S., 2023. A safe breeding ground: genetically improved maritime pine for growth and stem form has more efficient but not more vulnerable xylem. Tree Physiology, 43 (3): 366-378. DOI: https://doi.org/10.1093/treephys/tpac125.
Swedjemark, G., Karlsson, B., 2006. Mycelial growth and exclusion of Heterobasidion parviporum inoculated in branches of 15-year-old Picea abies clones. Forest Pathology, 36 (3): 209-214. DOI: https://doi.org/10.1111/j.1439-0329.2006.00452.x.
Swedjemark, G., Karlsson, B., Stenlid, J., 2007. Exclusion of Heterobasidion parviporum from inoculated clones of Picea abies and evidence of systemic induced resistance. Scandinavian Journal of Forest Research, 22 (2): 110-117. DOI: https://doi.org/10.1080/02827580701262774.
Swedjmark, G., Stenlid, J., 1997. Between-tree and between-isolate variation for growth of s-group Heterobasidion annosum in sapwood of Picea abies cuttings. Canadian Journal of Forest Research, 27 (5): 711-715. DOI: https://doi.org/doi.org/10.1139/x96-191.
Tereshchenko, L., 2011. Rezul’taty doslidzhennya pershykh v Ukrayini vyprobnykh kul’tur sosny zvychaynoyi. (Results of research of the first in Ukraine Scots pine progeny tests). Lisivnytstvo i Ahrolisomelioratsiya/Forestry and Forest Melioration, 118: 128-136.
Tereshchenko, L., Dyshko, V., 2019. Rezultaty 20-richnykh vyprobuvan kandydativ u sorty-populyatsiyi syntetychni sosny zvychaynoyi v umovakh DP Zmiyivske LH na Kharkivshchyni. (Results of 20-year testing of candidates to synthetic varieties-populations of Scots pine in conditions of Zmiyivske Forestry Enterprise in Kharkiv Region). Lisivnytstvo i Ahrolisomelioratsiya/Forestry and Forest Melioration, 134: 33-42.
Von Weissenberg, K., 1973. Indirect selection for resistance to fusiform rust in loblolly pine. Acta Forestalia Fennica, 46: 134. DOI: https://doi.org/10.14214/aff.7568.
Von Weissenberg, K., 1975. Variation in relative resistance to spread of Fomes annosus in four clones of Picea abies. European Journal of Forest Pathology, 5 (2): 112-117. DOI: https://doi.org/10.1111/j.1439-0329.1975.tb00452.x.
Witzell, J., Martín, J.A., 2008. Phenolic metabolites in the resistance of northern forest trees to pathogens-past experiences and future prospects. Canadian Journal of Forest Research, 38 (11): 2711-2727. DOI: https://doi.org/10.1139/X08-112.